全文获取类型
收费全文 | 104篇 |
免费 | 7篇 |
出版年
2022年 | 1篇 |
2021年 | 1篇 |
2019年 | 1篇 |
2018年 | 4篇 |
2017年 | 1篇 |
2016年 | 3篇 |
2015年 | 5篇 |
2014年 | 3篇 |
2013年 | 7篇 |
2012年 | 5篇 |
2011年 | 4篇 |
2010年 | 2篇 |
2009年 | 1篇 |
2007年 | 4篇 |
2006年 | 2篇 |
2005年 | 4篇 |
2004年 | 4篇 |
2003年 | 6篇 |
2001年 | 5篇 |
1999年 | 11篇 |
1998年 | 5篇 |
1997年 | 5篇 |
1996年 | 2篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1992年 | 3篇 |
1990年 | 2篇 |
1989年 | 1篇 |
1987年 | 4篇 |
1986年 | 3篇 |
1985年 | 2篇 |
1984年 | 2篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1973年 | 1篇 |
1874年 | 1篇 |
排序方式: 共有111条查询结果,搜索用时 15 毫秒
31.
Background
Adverse drug reactions (ADRs) are now recognized as an important cause of hospital admissions, with a proportion ranging from 0.9–7.9%. They also constitute a significant economic burden. We thus aimed at determining the prevalence and the economic burden of ADRs presenting to Medical Emergency Department (ED) of a tertiary referral center in India 相似文献32.
The scientific and medical community remains skeptical regarding the efficacy of nutrition for osteoarthritis despite their
broad acceptation by patients. In this context, this paper systematically reviews human clinical trials evaluating the effects
of nutritional compounds on osteoarthritis. We searched the Medline, Embase, and Biosis databases from their inception to
September 2005 using the terms random, double-blind method, trial, study, placebo, and osteoarthritis. We selected all peer-reviewed
articles reporting the results of randomised human clinical trials (RCTs) in osteoarthritis that investigated the effects
of oral interventions based on natural molecules. Studies on glucosamine and chondroitin sulfate were excluded. The quality
of the RCTs was assessed with an osteoarthritic-specific standardised set of 12 criteria and a validated instrument. A best-evidence
synthesis was used to categorise the scientific evidence behind each nutritional compound as good, moderate, or limited. A
summary of the most relevant in vitro and animal studies is used to shed light on the potential mechanisms of action. Inclusion criteria were met by 53 RCTs out
of the 2,026 identified studies. Good evidence was found for avocado soybean unsaponifiables. Moderate evidence was found
for methylsulfonylmethane and SKI306X, a cocktail of plant extracts. Limited evidence was found for the Chinese plant extract
Duhuo Jisheng Wan, cetyl myristoleate, lipids from green-lipped mussels, and plant extracts from Harpagophytum procumbens. Overall, scientific evidence exists for some specific nutritional interventions to provide symptom relief to osteoarthritic
patients. It remains to be investigated whether nutritional compounds can have structure-modifying effects. 相似文献
33.
Kolodgie FD Narula J Guillo P Virmani R 《Apoptosis : an international journal on programmed cell death》1999,4(1):5-10
Intimal cell death has been a recognized feature of advanced atherosclerotic disease. With the advent of DNA
in situ
end labelling and/or ultrastructural techniques, recent findings suggest that cells of an atheroma undergo programmed cell death or apoptosis. The pathophysiologic relevance of apoptosis in atherosclerotic disease is debatable. Apoptotic cell death may influence lesion progression and thus reduce overall plaque burden. Alternatively, apoptosis may prove a means of quenching the inflammation, converting cellular-rich lesions to so-called stable fibrous hypocellular plaques or conversely weaken the fibrous cap causing plaque rupture, a major cause of acute coronary syndromes. Apoptotic cells within plaques are typically macrophages, smooth muscle cells and T-cells and the frequency of death varies in the different regions of the lesion. The precise signalling pathways of apoptosis in plaques are unknown. There is however, some evidence that production of immune cytokines may promote apoptosis through activation of the Fas ligand-mediated death pathway. Genetic signals that regulate apoptosis in the atheroma, at least in smooth muscle cells, may involve the tumour suppressor genes p105
RB
and p53. Further studies as to the relevance of apoptosis in acute coronary syndromes and potential mechanisms are emerging. 相似文献
34.
Mucins comprise an important class of tumor-associated antigens. The objectives of the present study were (a) to establish an in vitro model system using human non-small cell lung adenocarcinoma cell lines NCIH650 and NCIH2077 (b) provide evidence that these cell lines secrete mucin in culture conditions and (c) investigate the effects of select secretagogues on mucin secretion. The cell lines were established in ACL-4 medium containing several growth factors and retinoic acid and 5% fetal calf serum. The high molecular weight glycoconjugates secreted in the culture medium were purified by ammonium sulfate precipitation and Superose 6 and Superose 12 FPLC chromatography. The purified high molecular weight glycoconjugate fraction and the carcinoma cells were shown to have mucin by dot blot, Western blot and immunohistochemical analysis, respectively, using specific antibodies to purified major mucin, HTM-1. Also, incorporation experiments with mucin precursor 3H-glucosamine demonstrated that the cells indeed synthesize high molecular weight mucins. The effects of secretagogues such as, 8-bromocyclic AMP, ionomycin, phorbol-12-myristate-13-acetate and neutrophil elastase on mucin secretion were also investigated. Only 8-bromocyclic AMP and neutrophil elastase influenced mucin secretion. These studies provided strong evidence that the lung adenocarcinoma cell lines secrete high molecular weight mucins in culture conditions and only two of the four tested secretagogues significantly increased mucin secretion. Thus, this in vitro model system may be useful in determining alterations in mucin structure, if any, in lung adenocarcinomas as well as in studying the regulation of mucin gene expression. 相似文献
35.
Molecular diversity and multilocus organization of the parental lines used in the International Rice Molecular Breeding Program 总被引:13,自引:0,他引:13
Yu SB Xu WJ Vijayakumar CH Ali J Fu BY Xu JL Jiang YZ Marghirang R Domingo J Aquino C Virmani SS Li ZK 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2003,108(1):131-140
One hundred and ninety three parental lines obtained from 26 countries for an international rice molecular breeding program were evaluated using 101 well-distributed simple sequence repeat (SSR) markers. An overall genetic diversity of 0.68 and an average of 6.3 alleles per locus were revealed, indicating a high level of genetic variation in these lines. Cluster analysis of the 193 accessions showed three major groups and nine subgroups. Group I corresponded to the classical indica subspecies, whereas groups II and III belong to the japonica subspecies. Indica and japonica differentiation accounted for only 6.5% of the total variation in the entire sample and 93.5% was due to within-subspecies diversity. Differentiation among eco-geographic regions accounted for 24% of the diversity within the subspecies. Larger amounts of the eco-geographical differentiation were resolved within japonica than within indica. The largest indica-japonica differentiation based on the single locus level was detected by markers on chromosomes 9 and 12, while the smallest differentiation was detected by markers on chromosomes 4 and 8. Furthermore, genetic differences at the single-locus and two-locus levels, as well as components due to allelic and gametic differentiation, were revealed between indica and japonica and among the main geographic regions. The multilocus analysis in genetic diversity showed a higher proportion of variation caused by predominant non-random associations of different loci within and among the classified subspecies and geographic subdivisions. The results suggest that selection for eco-geographical adaptation on multilocus associations was largely responsible for the maintenance of the extensive variation in the primary gene pool of rice.Communicated by Q. ZhangS.B. Yu and W.J. Xu contributed equally to the work 相似文献
36.
37.
Harish C. Pant Mridulika Virmani Paul E. Gallant 《Biochemical and biophysical research communications》1983,117(2):372-377
Calcium-dependent protease activity capable of degrading a number of endogenous proteins was found in rat red blood cell membranes. This protease activity, like that found in human red blood cells, was activated by low concentrations of calcium, but in the rat red blood cells, unlike the human red blood cells, calcium-activated protease activity was membrane-bound. A number of endogenous membrane-bound proteins were degraded after the addition of calcium to the membranes. These included spectrin bands 1 and 2 as well as bands 3, 2.1, and 2.2. No calcium-induced aggregation (transglutaminase activity) was noted in the rat red blood cell membranes. 相似文献
38.
Beneficial effect of fluorocarbon emulsion media on the function of neuromuscular preparations in vitro 下载免费PDF全文
The effects of liquid fluorocarbons as bathing media were determined by use of in vitro neuromuscular preparations. Rat hemidiaphragms were bathed in either oxygenated fluorocarbon (FC) emulsion or standard oxygenated Krebs solution. Contractile force in response to simple supramaximal nerve stimuli as well as to high frequency stimulation was greater, while twitch:tetanus ratio was smaller in FC emulsion. With such medium, post-tetanic potentiation of contraction was also more consistently observed. Indirectly stimulated diaphragms survived longer in FC emulsion. After cessation of oxygenation, oxygen tension (ρO(2)) of the medium declined more rapidly with Krebs than with FC emulsion; ρO(2) directly correlated with force of contraction. Similarly, in the chick biventer cervicis preparation, FC emulsion enhanced nerve-stimulated force of contraction; returning the preparation to standard Krebs solution reversed this phenomenon. Dose-resonse curves of muscle contraction in response to acetycholine and KCl administration were shifted upward during FC emulsion superfusion. Frequency of miniature endplate potentials was lower in FC emulsion than that observed in Krebs solution, measured from the same cell of the rat diaphragm. Resting membrane potentials were also greater in muscle cells sampled from FC emulsion-bathed preparations. These data suggest that FC emulsion is superior to standard Krebs solution as a bathing medium for in vitro neuromuscular preparations by virtue of the high solubility of oxygen in it. 相似文献
39.
40.
A. W. Julfiquar S. S. Virmani A. L. Carpena 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1985,70(6):671-678
Summary A representative group of 100 elite lines, 67 of which are restorers and 33 maintainers, from 68 crosses made at IRRI and
18 improved varieties from five countries were studied, using Mahalonobis, D2-statistic and canonical analysis, to understand the nature and magnitude of divergence and to assess the importance of a
set of quantitative characters related to yield in genetic differentiation. The 100 genotypes were grouped into 13 clusters.
There were three single variety clusters and the number of lines in the remaining clusters ranged from 2–36. Canonical vector
values indicated the importance of yield followed by 1,000-grain weight, days to maturity and plant height in primary as well
as in secondary differentiation. Results showed that yield, number of tillers per plant, days to maturity and 1,000-grain
weight contributed largely to the divergence. There were no indications of a relationship between geographical diversity and
genetic diversity in the present study. Disposition of IRRI developed maintainers and restorers into various clusters indicated
the presence of large amounts of diversity within the IRRI elite lines which suggested that these materials could be used
in crossing programs to produce heterotic F1 hybrids. Crossing of maintainers and restorers among the highly diverse groups was suggested as it may produce F1S that will give higher magnitudes of heterosis.
Part of M.S. Thesis of the senior author 相似文献